Table 1.
Summary of bchs alleles and phenotypes
| Genotype | Mean life span | SD | pvalue with WT | BCHS expression | Increase in IUCP* | Ubiquitin aggregates | APPL aggregates |
|---|---|---|---|---|---|---|---|
| Df(dsf3)/Df(clot7)bchs1 | 18.2 n = 190 | 6.3 | <0.0001 | 0 | 22.8 | + | + |
| Df(dsf4)/Df(clot7)bchs2 | 19.6 n = 136 | 6.5 | <0.0001 | 0 | nd | + | + |
| bchs3402Cy011/Df(clot7) | 19.1 n = 209 | 5.6 | <0.0001 | 0 | 16 | + | + |
| bchs4EP(2)2299/Df(clot7) | 21.7 n = 161 | 7.9 | <0.0001 | ++/0 | 6.9 | + | + |
| bchs4EP(2)2299/EP(2)2299 | 22.9 n = 135 | 8.3 | <0.0001 | ++/0 | nd | + | + |
| bchs5/bchs5 | 21.5 n = 99 | 8.5 | 0.00021-159 | nd | nd | + | nd |
| bchsrev1/bchsrev1 | 27.2 n = 81 | 7.7 | 0.811-159 | nd | nd | 0 | nd |
| Wild-type (Canton S) | 32 n = 123 | 9.1 | 1 | ++++ | 0 | 0 | 0 |
| 402CyO/Df(clot7) genotype control | 32.5 n = 151 | 5.8 | 0.67 | ++++ | nd | 0 | 0 |
| dsf1/Df(clot7) regional control | 31.5 n = 84 | 8.3 | 0.62 | +++ | nd | 0 | 0 |
Insoluble ubiquitin conjugated proteins.
The mean life span for each genotype is indicated, as are SDs andp value (Student t Test). Comparisons with wild-type (Canton S) and
F1-159: note that pvalues were calculated from wild-type controls aged at the same time and under the same conditions (average = 27.6, SD = 11.9, andn = 73). The + and 0 symbols used for BCHS expression summarize the Northern and Western analyses of the message and/or protein. The +/0 symbols used forbchs4 allele reflect the presence of the protein in young adults and its absence in aged individuals. The relative levels of ubiquitin and APPL aggregate formation detected by confocal (starting at 10 d of age) are summarized by + (presence) or 0 (absence) symbols. Of the three independent experiments done to examine levels of insoluble ubiquitin-conjugated proteins (IUCP), the representative Western blot in Figure 6 was used to calculate the fold increase after correcting for load (histone 2B) and normalization to wild-type controls.