Fig. 2.

Structure of Slit2 D2 bound to Robo1 Ig1. Ig1 is in green; Slit2 D2 N- and C-terminal caps are in purple and blue, respectively; LRRs 1–6 are in orange; and the disulfide bridges are in yellow. Interacting residues are shown in stick representation. (A) Ribbon diagram of the complex in two orthogonal orientations. (B) Electrostatic region of the Slit2 D2-Ig1 interface. (C) Hydrophobic region of the Slit2 D2-Ig1 interface. (D) Relative Slit2 D2-binding capacity of Robo1 Ig1 variants. Results are expressed as maximal Slit2 D2-binding, normalized with respect to the maximal Slit2 D2-binding capacity of wild-type Robo1 Ig1.