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Kinetic and dissociation constants and Slit2 D2-binding capacity of Robo1 constructs
| Construct | Robo1 Ig1–2 | Robo1 Ig1 | Robo1 Ig2 |
|---|---|---|---|
| Kar, M−1s−1 | 5.5 × 105 | 4.4 × 105 | ND |
| Kdr, s−1 | 4.5 × 10−3 | 3.5 × 10−3 | ND |
| Kd = Kdr/Kar, nM | 8.2 | 8.0 | ND |
| Relative Slit2 D2 binding* | 1 | 0.7 | 0.0 |
ND, not determined.
*The maximal Slit2 D2-binding capacity (Rmax) of the constructs was normalized vs. the maximal Slit2 D2-binding capacity of Robo1 Ig.
