Table 1.
Vector | Promoter 1 | Reporter 1 | Target | Promoter 2 | Reporter 2 | Target |
pEGFP-bPrP | CMV | EGFP | bPRNP | |||
pGL3-bPrP | SV40 | Fire-fly luciferase | bPRNP | |||
pFluc-bPrP-Rluc | SV40 | Fire-fly luciferase | bPRNP | HSV-TK | Renilla luciferase* | |
pFluc-Rluc-bPrP | SV40 | Fire-fly luciferase* | HSV-TK | Renilla luciferase | bPRNP | |
pTKFluc-bPrP-Rluc | HSV-TK | Fire fly luciferase | bPRNP | HSV-TK | Renilla luciferase* |
*, used as the internal control.