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. 2007 Jul 26;7:44. doi: 10.1186/1472-6750-7-44

Table 3.

Structure of cloning sites of siRNA expression vectors

Vector Promoter Sequence of cloning site
piGENE hU6 hU6 CACCGTGAGCAGGTGTAAAGCCACCATGGAAGACACCTGCCAAC TTTTTTCAATTGGTCGACCTGCAGGCATGCAAGCTTa
piGENE CACC-S/K hU6 CACCGAGCTCAGACTCGATATCGGTACCb
piGENE S/K hU6 GAGCTCAGACTCGATATCGGTACCc
piGENE tRNA tRNA GAGCTCCAGATCTAATGCGGCCGCTTAGGTACCATAGATATCTTTTTTTCTGCAGGCATGCAAGCTTd

a: Three Bsp MI sites are underlined. b and c: Sac I, Bgl II, Eco RV, and Kpn I sites are underlined. d: Sac I, Bgl II, Not I, Kpn I, Eco RV, Pst I, and Hind III sites are underlined. T repeats, RNA pol III terminator signal, are italicized.