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Effect of ThDP concentration on the activity of AThTP-synthesizing enzyme at fixed concentrations of ADP (1 mM) and Mg2+(10 mM). The continuous line was obtained by nonlinear regression using the Michaelis-Menten equation with an apparent Km of 4.2 ± 0.5 mM. Inset shows the Hanes plot of the data and the line was obtained by linear regression. The results are expressed as mean ± SD for 3 experiments.
