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Partial purification of AThTP-synthesizing enzyme from E. coli
| Fraction | Volume (ml) | Total protein (mg) | Total activity (pmol.min-1) | Specific activity (pmol.min-1.mg-1) | Purification factor |
| Extract | 32.0 | 99.2 | 436.5 | 4.4 | 1 |
| Heat treatment | 31.0 | 71.3 | 370.8 | 5.2 | 1.2 |
| Sephadex G-200: | |||||
| Peak I (maximum) | 4.0 | 0.68 | 63.4 | 93.2 | 21.2 |
| Peak II (maximum) | 4.0 | 0.94 | 75.4 | 80.2 | 18.2 |
The purification factor was calculated by comparing the activity in a given fraction to the activity found in the extract.
