Table 1.
Transfection activity and toxicity of different amphiphiles examined in COS-7 cells
| Amphiphile | Concentration,* μM | Toxicity,† % of nonsurviving cells | Transfection efficiency,‡ arbitrary unit |
|---|---|---|---|
| DOTMA/DOPE | 36 –71 | 30 –40 | 1 |
| SAINT-1/DOPE | 38 –77 | 10 –20 | 1 |
| SAINT-2/DOPE | 15 –38 | 0 –25 | 8 |
| SAINT-3/DOPE | 8 –15 | 0 –20 | 2.5 |
| SAINT-4/DOPE | 15 –38 | 0 –30 | 12 |
| SAINT-5/DOPE | 15 –38 | 0 –10 | 0.6 |
| SAINT-7/DOPE | 5 –38 | 0 –40 | 10 |
| SAINT-8/DOPE | 5 –38 | 0 –30 | 3 |
| SAINT-9/DOPE | 38 –77 | 0 | 4 |
| SAINT-12/DOPE | 5 | 5 | 1.2 |
| SAINT-13/DOPE | 5 –10 | 25 –40 | 2.5 |
*
The concentration given is the concentration range at which the amphiphile is active.
†
The toxicities given here are the toxicities measured at the active concentration range, i.e., at the highest and lowest concentration given, respectively.
‡
The different transfection efficiencies reflect the maximal efficiencies found within the amphiphile concentration range given and are expressed relative to the efficiency obtained with DOTMA/DOPE (=1). Transfection efficiencies were determined in at least three different experiments. Average values are given. (Error ± 10%.)