Fig. 2.

Expression of cdc25⁺ and spo6⁺ during meiosis and effects of mei4⁺ overexpression or mutation. (A) Cells of the indicated genotypes (WT, HM1307; mei4, HM2163; mik1 wee1, HM605; mei4 mik1 wee1, HM1959; and mei4⁺-OP, HM4582) were subjected to synchronous induction of meiosis as described in Fig. 1. Samples were collected at the indicated times thereafter and subjected to Northern blot analysis of cdc25⁺ and spo6⁺ mRNA. Transcripts of cdc2⁺ were analyzed as a loading control. (B) Cells expressing GFP-tagged Cdc25p (WT cdc25⁺-GFP, HM4732; mei4 cdc25⁺-GFP, HM4833; and mei4⁺-OP cdc25⁺-GFP, HM4735) were treated as in A and subjected to immunoblot analysis with antibodies to GFP and to α-tubulin (loading control). (C) Cells of the indicated genotypes were induced to undergo meiosis as in Fig. 1. After incubation for 24 h at 34°C, the cells were examined with staining with iodine.