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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: J Immunol. 2007 Jun 1;178(11):7376–7384. doi: 10.4049/jimmunol.178.11.7376

FIGURE 5.

FIGURE 5

An herb-derived antioxidant, quercetin, attenuates H2O2-induced Hsp72 nuclear translocation, and Hsp72-HMGB1 interaction in macrophage cultures. RAW 264.7 cells transfected with pcDNA3.1-Hsp72 construct were pretreated with quercetin (50 μM) for 4 h and subsequently stimulated with H2O2 (0.125 mM) for 3 h. Subcellular localization of Hsp72 in cells was determined by immunocytochemistry (A) or Western blot (B). Results are representative of three experiments with similar results. Green, Hsp72; blue, nuclei. Original magnification, ×400). Ctrl, control cells; Q, + quercetin; H, + H2O2; QH, + quercetin + H2O2. In parallel experiments, whole-cell lysate was immunoprecipitated with Hsp72-specific Abs, and the precipitated complexes were immunoblotted with Hsp72- or HMGB1-specific Abs, respectively (C). IP, immunoprecipitation; IB, immunoblotting. Blot is representative of three experiments with similar results.

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