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. Author manuscript; available in PMC: 2007 Sep 12.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2007 Mar 1;67(3):870–878. doi: 10.1016/j.ijrobp.2006.10.030

Figure 4.

Figure 4

Figure 4

Time course analyses of endothelial cell apoptosis and proliferation. (a) Endothelial cell apoptosis as determined by CD31-TUNEL double-staining. The number of apoptotic endothelial cells was counted in 7-9 random fields at ×400 magnification. (b) Endothelial cell proliferation as determined by CD31-BrdU double-staining. The number of proliferating endothelial cells was determined in 18-25 random fields at ×200 magnification. (c) Representative staining for CD31 (red fluorescence), BrdU (green fluorescence), or both CD31 and BrdU (yellow fluorescence) in tumor tissue harvested from mice killed 7 days after irradiation.