Table 1.

Interactions between viral and cellular DED-containing proteins by yeast two-hybrid analysis

DNA-binding hybrid	Activation hybrid	Filter assay (intensity of blue color)	Liquid assay (β-gal activity, Miller units)
LexA-caspase-8 DED A + B	B42-E8	+++	163  ± 2.6
LexA-caspase-8 DED A + B	B42-E8 DED	++	16.3  ± 2.9
LexA-caspase-8 DED A + B	B42	—	7.5  ± 0.8
LexA-E8 DED	B42-caspase-8 DED A + B	++	44  ± 8.0
LexA-E8 DED	B42-caspase-8 DED B	+++	162  ± 44
LexA-E8 DED	B42-caspase-8 DED A	—	21  ± 2.0
LexA-E8 DED	B42	—	21  ± 3.3
LexA-E8 DED	B42-FADD	—	15  ± 0.5
LexA-E8 DED	B42	—	14  ± 1.5
LexA-MC159	B42-FADD	++++	6890  ± 1627
LexA-MC159	B42-caspase-8 DED A + B	—	31  ± 0.1
LexA-MC159	B42-caspase-8 DED B	+	61  ± 8.9
LexA-MC159	B42-caspase-8 DED A	—	39  ± 19
LexA-MC159	B42	—	27  ± 1.0

Data represent four independent experiments. ++++, Intense blue color; +++, strong blue color; ++, medium blue color; +, light blue color; —, white.