Table 1.
Interactions between viral and cellular DED-containing proteins by yeast two-hybrid analysis
| DNA-binding hybrid | Activation hybrid | Filter assay (intensity of blue color) | Liquid assay (β-gal activity, Miller units) |
|---|---|---|---|
| LexA-caspase-8 DED A + B | B42-E8 | +++ | 163 ± 2.6 |
| LexA-caspase-8 DED A + B | B42-E8 DED | ++ | 16.3 ± 2.9 |
| LexA-caspase-8 DED A + B | B42 | — | 7.5 ± 0.8 |
| LexA-E8 DED | B42-caspase-8 DED A + B | ++ | 44 ± 8.0 |
| LexA-E8 DED | B42-caspase-8 DED B | +++ | 162 ± 44 |
| LexA-E8 DED | B42-caspase-8 DED A | — | 21 ± 2.0 |
| LexA-E8 DED | B42 | — | 21 ± 3.3 |
| LexA-E8 DED | B42-FADD | — | 15 ± 0.5 |
| LexA-E8 DED | B42 | — | 14 ± 1.5 |
| LexA-MC159 | B42-FADD | ++++ | 6890 ± 1627 |
| LexA-MC159 | B42-caspase-8 DED A + B | — | 31 ± 0.1 |
| LexA-MC159 | B42-caspase-8 DED B | + | 61 ± 8.9 |
| LexA-MC159 | B42-caspase-8 DED A | — | 39 ± 19 |
| LexA-MC159 | B42 | — | 27 ± 1.0 |
Data represent four independent experiments. ++++, Intense blue color; +++, strong blue color; ++, medium blue color; +, light blue color; —, white.