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. 2007 Sep 14;3(9):e162. doi: 10.1371/journal.pgen.0030162

Figure 5. AOP2 Transcript Alters Metabolic Profiles, Content, and Gene Expression.

Figure 5

Wild-type Col-0 that is null for AOP2 and AOP3 was modified through the introduction of a functional AOP2 transcript from B. oleracea. All glucosinolate abbreviations are as described in Table S1.

(A) HPLC profile of aliphatic glucosinolates detected in foliar tissue of wild-type Col-0.

(B) HPLC profile of aliphatic glucosinolates detected in foliar tissue of Col-0 containing the functional AOP2 transcript.

(C) Average total foliar aliphatic glucosinolate content in Col-0 and Col-0::AOP2 is shown with standard error bars. Six plants per genotype were measured for total aliphatic glucosinolate content within an experiment, and the experiment was conducted twice to provide 32 total measurements. **p < 0.0001 as determined by ANOVA.

(D) Percentage increase in transcript levels in Col-0::AOP2 as compared with Col-0 is presented. RNA from 3 plants per genotype were individually hybridized to ATH1 Affymetrix arrays to obtain transcript levels for the aliphatic glucosinolate genes. ANOVA was used to test for significant differences between the two genotypes for the glucosinolate biosynthetic and regulatory genes via ANOVA with a false discovery rate of 0.05. Gray bars show transcripts significantly increased by the introduction of the AOP2 transcript. Nonsignificant changes are shown in white bars.