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Longitudinal phenotypic characterization of tetramer-positive CD3+ CD8+ T cells. Analysis of PBMCs was performed by 6-color flow cytometry in 2 ALL patients at defined time points after SCT. CD45RO, CD27, and CD57 phenotype of CD3+ CD8+ T-cell gated tetramer positive lymphocytes on samples from patient 1 is presented here. (A) CMVpp65495/HLA-A*0201+ CD8+ T cells. (B) WT1/HLA-A*0201+ CD8+ T cells
