Table 1.

Kinetic determination of VWF binding to ADAMTS13 (or variants) by SPR

VWF; KD, ×10−9 M (n)	VWF;*KD ×10−9 M (n)
FL-A13
50±9 (6)	83 ± 17 (6)
77±26 (4)†	—
delCUB
274±92 (6)	242 ± 73 (6)
468±131 (4)†	—
MDTC
No binding	337 ± 186 (6)
No binding†	—

The entries are means plus and minus a standard error.

FL-A13 is the full-length ADAMTS13; delCUB, the ADAMTS13 variant truncated after the eighth TSP1 repeat; MDTCS, the variant truncated after the spacer domain; n, the number of repeats performed; —, not applicable.

^*The VWF substrate was denatured at 37°C for 2 hours with 1.5 M quanidine HCl prior to binding experiments.

^†Data obtained from experiments performed in presence of 10 mM EDTA.