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. 2007 Aug 1;65(3):607–624. doi: 10.1111/j.1365-2958.2007.05806.x

Fig. 3. The product of pglA is required for disaccharide modification of PilE.

Fig. 3

A. ESI mass spectrum over a range of 180–1800 m/z; strain KS141 (pglA::kan). The major oxonium ion species detected is boxed; DATDH (m/z 229.1).

B. Deconvoluted molecular weight spectrum from intact PilE ESI-MS analyses. The two major peaks at m/z 17530.0 and m/z 17653.0 represent PilE modified with DATDH in conjunction with one and two PE groups respectively.

C. CID MS/MS spectrum of the major oxonium ion species at 229.1 m/z (boxed) generated by in-source fragmentation. A complete list of all oxonium ion species with m/z-values and corresponding molecular weight values of all PilE species are found in Table S1.