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. 2007 Aug 1;65(3):607–624. doi: 10.1111/j.1365-2958.2007.05806.x

Fig. 8. The product of pglI is required for acetylation of the PilE glycan.

Fig. 8

A. Deconvoluted molecular weight spectrum from intact PilE ESI-MS analysis showing the pilin species found in a pglI-null mutant (strain KS144, pglI::kan). The two major peaks at 17 691.5 and 17 814.5 Da represent PilE modified with HexDATDH in conjunction with one and two PE groups respectively.

B. ESI mass spectrum over a range of 180–1800 m/z. Oxonium ions for DATDH (m/z 229.1) and HexDATDH (m/z 391.2; boxed) are seen. A complete list of all oxonium ion species with m/z-values and corresponding molecular weight values of all PilE species are found in Table S1.