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. 2007 Aug 1;65(3):700–713. doi: 10.1111/j.1365-2958.2007.05820.x

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© 2007 The Authors Journal compilation © 2007 Blackwell Publishing Ltd

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Fig. 7 — Top. Autoradiography of SDS-PAGEs after electrophoresis of the radiolabelled proteins synthesized in a S. solfataricus in vitro translation systems programmed with increasing amounts of aIF2/5B mRNA and fixed amounts of a leaderless (α-fucosidase) or a leadered (104) mRNA. Lane 1: 10 pmol of α-fuc or 10 pmol of 104 mRNA in the absence of aIF2/5B mRNA; Lanes 2–4: same with 10, 20 and 40 pmol, respectively, of aIF2/5B mRNA. Bottom. Curve obtained by quantifying with a Molecular Dynamics Phosphoimager the radioactivity retained in the bands as shown in the top panel. The relative increment on the y-axis is the ratio between radioactivity values in the absence of added aIF2/5B mRNA and values measured in the presence of the indicated amounts of aIF2/5B mRNA.