Table 1. Top 15 candidate housekeeping genes identified in 13,629 samples.
Gene symbol | name | mean | SD | CV (%) | MFC | rank |
RPS13 | ribosomal protein S13 | 12.82 | 0.33 | 2.59 | 1.61 | 1 |
RPL27 | ribosomal protein L27 | 12.70 | 0.35 | 2.73 | 1.41 | 2 |
RPS20 | ribosomal protein S20 | 12.81 | 0.37 | 2.90 | 1.67 | 3 |
RPL30 | ribosomal protein L30 | 13.08 | 0.42 | 3.22 | 1.99 | 4 |
RPL13A | ribosomal protein L13A | 13.01 | 0.43 | 3.29 | 1.83 | 5 |
RPL9 | ribosomal protein L9 | 12.95 | 0.44 | 3.36 | 1.68 | 6 |
SRP14 | signal recognition particle 14kDa | 11.45 | 0.40 | 3.46 | 1.48 | 7 |
RPL24 | ribosomal protein L24 | 12.50 | 0.46 | 3.65 | 1.54 | 8 |
RPL22 | ribosomal protein L22 | 11.94 | 0.44 | 3.68 | 1.91 | 9 |
RPS29 | ribosomal protein S29 | 12.86 | 0.47 | 3.69 | 1.93 | 10 |
RPS16 | ribosomal protein S16 | 12.48 | 0.47 | 3.73 | 1.62 | 11 |
RPL4 | ribosomal protein L4 | 12.43 | 0.47 | 3.76 | 1.63 | 12 |
RPL6 | ribosomal protein L6 | 12.22 | 0.46 | 3.76 | 1.65 | 13 |
OAZ1 | ornithine decarboxylase antizyme 1 | 11.88 | 0.45 | 3.78 | 1.51 | 14 |
RPS12 | ribosomal protein S12 | 12.90 | 0.49 | 3.82 | 1.99 | 15 |
CV, indicates the coefficient of variation and equals the standard deviation divided by the mean (expressed as a percentage). MFC, indicates the maximum fold change, i.e. the ratio of the maximum and minimum values observed within a dataset. The ranking is based upon three criteria: CV, a MFC<2 and a mean value lower then the maximum value with 2 standard deviation (SD) subtracted.