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. 2007 Sep 19;2(9):e913. doi: 10.1371/journal.pone.0000913

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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After each probe has been fit to the model, described in Material and Methods, the rescaled variables overlay the classical Langmuir adsorption isotherm, shown in dotted black. B) Distribution of Initial Slopes for HuEx [red boxplot] and U133Plus2 [blue boxplot] spiked probes. C) Local slopes as a function of concentration for HuEx [median red line and inter-quartile range shaded red] vs U133Plus2 [median blue line and inter-quartile range shaded blue]. Local slopes represent the fitted log2 fold-change for probes with true log2 fold-change of 1 as a function of the nominal concentration. D) Distribution of detection threshold for HuEx 1.0 [red boxplot] and U133Plus2 [blue boxplot] spiked probes. Detection threshold is referred to probe concentration where signal-to-noise ratio exceeds 3.

Inline graphic — After each probe has been fit to the model, described in Material and Methods, the rescaled variables overlay the classical Langmuir adsorption isotherm, shown in dotted black. B) Distribution of Initial Slopes for HuEx [red boxplot] and U133Plus2 [blue boxplot] spiked probes. C) Local slopes as a function of concentration for HuEx [median red line and inter-quartile range shaded red] vs U133Plus2 [median blue line and inter-quartile range shaded blue]. Local slopes represent the fitted log2 fold-change for probes with true log2 fold-change of 1 as a function of the nominal concentration. D) Distribution of detection threshold for HuEx 1.0 [red boxplot] and U133Plus2 [blue boxplot] spiked probes. Detection threshold is referred to probe concentration where signal-to-noise ratio exceeds 3.