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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Glycobiology. 2006 Aug 29;16(12):1219–1228. doi: 10.1093/glycob/cwl041

Fig. 5. Relative abundance of sialic acid derivatives isolated from human tear fluid.

Fig. 5

Seven human tear samples were hydrolyzed using sialidase from A. ureafaciens and individually analyzed by fluorometric HPLC. Shown is the relative abundance of individual sialic acids as a percentage of total sialic acid (values are mean ± SD, n=7). The presence of the H185 carbohydrate epitope in each sample (A-G) analyzed by fluorometric HPLC is confirmed by western blot (5-50 μg of total protein). The presence of two molecular weight bands migrating over 250 kDa may result from mucin polymorphism (individual mucin alleles are expressed co-dominantly), or the presence of different mucin gene products or glycoforms containing the H185 carbohydrate epitope in the sample.