Skip to main content
. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Glycobiology. 2006 Aug 29;16(12):1219–1228. doi: 10.1093/glycob/cwl041

Fig. 6. Identification of sialic acid derivatives after H185 antigen immunoprecipitation.

Fig. 6

The H185 antigen, which in HCLE cells is carried by the membrane-associated mucin MUC16 (Argueso et al., 2003), was immunoprecipitated from HCLE cultures as described in Materials and Methods. Sialic acids in the H185 mucin immunoprecipitate (H185 IP) were hydrolyzed using A. ureafaciens sialidase, labeled with DMB and analyzed by fluorometric HPLC. A control (no tear sample) was carried out to determine the sialic acid content of the antibodies used for immunoprecipitation. The efficiency of the H185 mucin immunoprecipitation was assessed by immunoblot (inset). The low molecular weight band corresponds to antibodies used in the mucin immunoprecipitation assay.