Figure 1.

Domains in the amino and carboxyl termini of REST are required, in part, to mediate repression of type II sodium channel reporter genes. The family of REST molecules expressed under control of the cytomegalovirus (CMV) promoter, in PC12 cells, is shown with respect to the presence of known motifs in the deduced primary structure of REST (top line). The dotted lines indicate the regions in REST that were deleted. A cartoon of the cotransfected reporter gene shows the positions of the type II repressor element 1 (RE1), type II sodium channel promoter (type II) and CAT gene (bottom line). Arrows indicate the start sites for transcription in the expression plasmids. On the right, the percent activity of CAT resulting from transfection of the different constructs is normalized to that from the vector alone, which is set to 100%. Standard errors of the mean and (in parentheses) the numbers of experiments are indicated.