Representative autoradiographs depicting α-[125I]-BTX binding in animals exposed to a standard choline diet (left column), a choline deficient diet (middle column) or a choline supplemented diet (right column) for 14 days. Choline supplemented animals exhibited significantly higher BTX binding in some brain regions compared to animals that consumed the standard choline or choline deficient diets. Cx 1–4, cerebral cortex layers 1–4; Cx 5–6, cerebral cortex layers 5–6; DG, dentate gyrus; En, endopiriform nucleus; Hi, hilus of the dentate gyrus; PH, posterior hypothalamus SC, superior colliculus; SO, stratum oriens; STh, subthalamic nucleus; VLG, ventrolateral geniculate nucleus.