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. 2007 Jul 25;35(15):5108–5119. doi: 10.1093/nar/gkm546

Figure 3.

Figure 3.

ABA-spermine photoincorporation into naked 5S rRNA at various concentrations of the photoprobe. Photoaffinity labeling was carried out in 15 μl of buffer 40 mM HEPES-KOH, pH 7.2, 6 mM magnesium acetate and 100 mM NH4Cl, also containing 174 pmol of 5S rRNA, and ABA-spermine at the indicated concentrations; (filled circles), specific binding; (open circles), non-specific binding estimated by performing photolabeling in the simultaneous presence of spermine in excess.