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Organization of the HIV-1 genomic RNA. The HIV-1 genomic RNA, the untranslated R, U5, U3 regions and all open reading frames are indicated. The secondary structures of the 5′ and 3′R domains are shown on top. To map the sequences involved in the 5′/3′ interaction we made transcripts that are truncated from the 3′ terminus by 100 nt steps. Their ability to form the 5′/3′ interaction is indicated by +/−.
