Figure 3.

Mapping the minimal sequences that form the 5′/3′ interaction. (A) The 5′ 1-kb transcript was used to generate a set of radiolabeled transcripts with 3′ 100 nt truncations. These transcripts were either incubated alone (left panel) or with non-radiolabeled 3′ 1-kb transcripts (right panel). The arrow indicates the 5′/3′ complex (B). The 3′ 1-kb transcript was used to generate a set of radiolabeled transcripts with 3′ 100 nt truncations. These transcripts were either incubated alone (left panel) or with non-radiolabeled 5′ 1-kb transcripts (right panel). The results are summarized in Figure 1 with +/− signs.