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Repression of LDLR expression by incubation with high sterol levels. (A) DiI-LDL assay on pEPHZ-LDLR clones, cl 1 and cl 2, in the presence of sterols shows a reduction of expression by 65 and 70%, respectively, when compared to the negative control. (B) Fluorescence microscopy analysis of the reporter gene EGFP shows that reduction of expression in the presence of sterol is LDLR specific as EGFP expression is not affected.
