Table 2.
Code | Type | Sequencea | CD Typeb,c | Tmd (ΔTm)e (°C) |
---|---|---|---|---|
d(T2G4T2) and related sequences | ||||
PG17 | All PO-DNA | PO-d(TTGGGGTT) | I | 66.0 |
PG18 | All PS-DNA | PS-d(TTGGGGTT) | I | 73.5(43) |
74.0 | ||||
PG19 | All PS-2′F-ANA | PS-d(TTGGGGTT) | I | 83.0 (+1.1) |
PG20 | PS-2′F-ANA-G | PS-d(TTGGGGTT) | I | 87.0 (+3.3) |
d(G4T4G4) and related sequences | ||||
PG21 | All PO-DNA | PO-d(GGGGTTTTGGGG) | II | 65(44) |
64.4 | ||||
PG22 | All PO-2′F-ANA | PO-d(GGGGTTTTGGGG) | I | 90.0 (+2.1) |
PG23 | G-syn | PO-d(GGGGTTTTGGGG) | I | 72.5 (+2.0) |
PG24 | G-anti | PO-d(GGGGTTTTGGGG) | II | 66.2 (+0.5) |
aCapital and bold letter: 2′F-ANA; PO: phosphate linkage; PS: phosphorothioate linkage.
bCD type I & II refer to the note in Table 1.
cdT2G4T2 and related sequences (PG17–20): phosphate-buffered saline (PBS buffer, pH 7.2 at 25°C), 137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8 mM Na2HPO4; strand concentration: 20 µM for both CD and Tm experiments. dG4T4G4 and related sequences (PG21–24): 10 mM sodium phosphate buffer, 0.1 mM EDTA, pH 7 and 200 mM NaCl; strand concentration: 10 µM.
ddT2G4T2 and related sequences (PG17–20): phosphate-buffered saline (PBS buffer, pH 7.2 at 25°C), 137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8 mM Na2HPO4; strand concentration: 20 µM; Tm data were generated from concentration-dependent CD spectra (Figure S5 and ‘Materials and methods’ section). dG4T4G4 and related sequences (PG21–24): 10 mM sodium phosphate buffer, 0.1 mM EDTA, pH 7 and 200 mM NaCl; strand concentration: 100 µM; Tm measurements were conducted at 295 nm wavelength.
eΔTm (°C) is the Tm change/2′F-ANA modification of PG18–20 or PG22–24 relative to the control PG18 (74.0°C) or PG21 (PG64.4°C), respectively.