Table 2.

CD and T_m data of d(T₂G₄T₂) and d(G₄T₄G₄) and related oligonucleotides

Code	Type	Sequencea	CD Typeb,c	Tmd (ΔTm)e (°C)
d(T2G4T2) and related sequences
PG17	All PO-DNA	PO-d(TTGGGGTT)	I	66.0
PG18	All PS-DNA	PS-d(TTGGGGTT)	I	73.5(43)
				74.0
PG19	All PS-2′F-ANA	PS-d(TTGGGGTT)	I	83.0 (+1.1)
PG20	PS-2′F-ANA-G	PS-d(TTGGGGTT)	I	87.0 (+3.3)
d(G4T4G4) and related sequences
PG21	All PO-DNA	PO-d(GGGGTTTTGGGG)	II	65(44)
				64.4
PG22	All PO-2′F-ANA	PO-d(GGGGTTTTGGGG)	I	90.0 (+2.1)
PG23	G-syn	PO-d(GGGGTTTTGGGG)	I	72.5 (+2.0)
PG24	G-anti	PO-d(GGGGTTTTGGGG)	II	66.2 (+0.5)

^aCapital and bold letter: 2′F-ANA; PO: phosphate linkage; PS: phosphorothioate linkage.

^bCD type I & II refer to the note in Table 1.

^cdT₂G₄T₂ and related sequences (PG17–20): phosphate-buffered saline (PBS buffer, pH 7.2 at 25°C), 137 mM NaCl, 2.7 mM KCl, 1.5 mM KH₂PO₄, 8 mM Na₂HPO₄; strand concentration: 20 µM for both CD and T_m experiments. dG₄T₄G₄ and related sequences (PG21–24): 10 mM sodium phosphate buffer, 0.1 mM EDTA, pH 7 and 200 mM NaCl; strand concentration: 10 µM.

^ddT₂G₄T₂ and related sequences (PG17–20): phosphate-buffered saline (PBS buffer, pH 7.2 at 25°C), 137 mM NaCl, 2.7 mM KCl, 1.5 mM KH₂PO₄, 8 mM Na₂HPO₄; strand concentration: 20 µM; T_m data were generated from concentration-dependent CD spectra (Figure S5 and ‘Materials and methods’ section). dG₄T₄G₄ and related sequences (PG21–24): 10 mM sodium phosphate buffer, 0.1 mM EDTA, pH 7 and 200 mM NaCl; strand concentration: 100 µM; T_m measurements were conducted at 295 nm wavelength.

^eΔT_m (°C) is the T_m change/2′F-ANA modification of PG18–20 or PG22–24 relative to the control PG18 (74.0°C) or PG21 (PG64.4°C), respectively.