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. 2007 Aug 7;35(15):e100. doi: 10.1093/nar/gkm575

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — CTD did not demonstrate a 3′–5′ exonuclease activity. The substrate dsDNA whose 3′-terminus was blocked or unblocked with LNA was incubated with CTD (A) or KOD polymerase (B) and subjected to electrophoresis on an 11% polyacrylamide sequencing gel. Lanes 1–4: unblocked substrates were digested with 0, 50, 100 or 200 nM CTD, respectively; lanes 5–8: blocked substrates were digested with 0, 50, 100 or 200 nM CTD, respectively; lanes 9 and 10: unblocked substrates were digested with 0 or 5 nM KOD polymerase; lanes 11 and 12: blocked substrates were digested with 0 or 5 nM KOD polymerase. The length of the product fragments (bases) are indicated by numbers to the right of panel A.