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. 2007 Jul 26;35(15):5154–5164. doi: 10.1093/nar/gkm543

Figure 3.

Figure 3.

(a) Synthetic siRNAs can reduce the ability of a shRNA to down-regulate its target. A 10 nM concentration of each synthetic siRNA (siH3, siH6 and siH1) was co-transfected with the firefly luciferase target (FLT) independently or together with the anti-firefly shRNA (Luc). The firefly luciferase units were normalized against Renilla luciferase, which was used as transfection control. (b) Over-expression of Exportin-5 decreases shRNA competition but does not eliminate competition among interfering RNAs. Twenty nanomolar of each synthetic siRNA (siH3, siH6, siH1) or 50 ng of each shRNA construct (shL, shSII, shSI) were co-transfected with the luciferase target and the Luc shRNA with or without 100 ng of the Exportin-5-expressing plasmid (Exp-5) as indicated in the figure (+Exp-5, -Exp-5). Firefly luciferase units were calculated as previously described.