Figure 2.

Native gel analysis of competitive reconstitutions. Competitor DNAs are: lanes 1–4, RsaI-digested genomic fragments; lane 5, core particle DNA. Tracer DNAs are: lanes 1 and 5, core particle DNA; lane 2, pool of synthetic random DNA; lanes 3 and 4, individual cloned molecules from the synthetic random pool. S, kinased 100-bp ladder. Lanes 6 and 7, two particular cloned DNA molecules having relatively high (lane 6) or lower (lane 7) affinity for histone octamer in nucleosome reconstitution. N and D, nucleosomes and naked DNA, respectively.