Figure 7.

Effect of pollination on transcript levels of SlGA2ox1, -2, -3, -4, and -5 genes. Semiquantitative transcript analysis was carried out by RT-PCR, as described in “Materials and Methods,” using total RNA from unpollinated (days 0, 5, 10, and 20) and pollinated (days 5, 10, and 20) ovaries. E, Entire ovary; P, pericarp; S, seeds. For each gene, figures below the blots mean normalized values of gene expression versus that of Actin (used as an internal control; expression of unpollinated day 5 and 10 ovaries set at 1.0 for SlGA2ox1, -3, and -4, of pollinated day 5 ovaries for SlGA2ox2, and seeds from day 10 pollinated ovaries for SlGA2ox5). Data come from a representative experiment out of two biological replicates with similar results.