Figure 3.

DMI1 and dmi1-2 mutant proteins alter Li⁺ and Na⁺ sensitivity of the G19 (ena1-4Δ) yeast strain. A, Drop assays. Log-phase yeast cultures (1 × 10⁷ cells mL⁻¹) were diluted 10, 100, and 1,000-fold, and spotted onto SC (Gal) agar plates containing the indicated concentrations of Na⁺ or Li⁺. Drop assays were repeated three times with similar results. B, Na⁺ accumulation assay. G19 yeast transformed with pYES2 (black circles) or pYES2:DMI1 (white circles) was grown in SC (Gal) medium containing the indicated concentrations of Na⁺. Data are the means ± sem of three independent experiments.