Northern-blot analysis of transformed tobacco. RNA was extracted from leaves of tobacco plants, separated on a gel, transferred to a nylon membrane, and challenged with 32P-labeled AgMaT2 cDNA. For each plant type, two individual plants were used. Lanes C: control plants; lanes B5: B5 plants; lanes B6: B6 plants; lanes B4: B4 plants. Top row, Autoradiographs after challenging the membrane with the labeled probe; bottom row, staining of rRNA.