Fig. 4.

In situ hybridization analysis of Dmbx1 mRNA in the mesencephalon, pons, and medulla of E15.5 mouse fetuses. The lateral parabrachial nucleus (LPB) around the superior cerebellar peduncle (asterisks in A and B) is strongly labeled with silver grains. IS, isthmus; V4, fourth ventricle. The rostral NTS (rNTS) shows signals, whereas the caudal NTS (cNTS) does not (C–F). (E and F) Significant signals in the medulla also are distributed in the dorsal motor nucleus of vagus (DMV) and the reticular nucleus (RET). B, D, and F are dark-field images of A, C, and E, respectively. (Scale bars, 500 μm.)