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. 2007 Sep 26;2(9):e924. doi: 10.1371/journal.pone.0000924

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Mycoplasma pneumoniae (M.p.) DNA, at 1 ng/ul, was serially diluted and amplified with primer pools 1A (3 M.p. amplicons), 1B (2 M.p. amplicons), and 1C (1 M.p. amplicon). Panels in (A) illustrate the sensitivity obtained with the 3 pools at M.p. DNA dilutions of 1∶1,000 (left) and 1∶10,000 (right). Average signal intensities in picoamps for each set of amplicon probes (vertical bars) are shown at the left. The horizontal bar within graphs indicates the assay cutoff (mean 3±SDM). The PCR amplicons analyzed for each pathogen are listed in panel (B) and the three M.p. amplicons are indicated by thick underline.