Table 4.
Effect of GSK-3 inhibitors on spindle morphology
| Parameter | Control | SB-415286 | AR-A014418 | 1-Aza kenpaullone | CHIR99021 |
| Pole-Pole Distance (μm) | 8.39 ± 0.45 | 9.45 ± 0.37 | 10.36 ± 0.57 | 9.06 ± 0.39 | 8.18 ± 0.33 |
| (22)* | (20) | (19) | (20) | (18) | |
| - | p > 0.05§ | p < 0.05 | p > 0.05 | p > 0.05 | |
| Tubulin Intensity (midzone/cell max) | 0.39 ± 0.02 | 0.21 ± 0.01 | 0.25 ± 0.01 | 0.24 ± 0.01 | 0.26 ± 0.02 |
| (15)* | (20) | (19) | (20) | (18) | |
| - | p < 0.001 | p < 0.01 | p < 0.001 | p < 0.05 | |
| Interkinetochore Distance (μm) | 0.91 ± 0.04 | 1.04 ± 0.03 | 1.05 ± 0.04 | 0.93 ± 0.02 | 0.92 ± 0.02 |
| (40, 4) | (33, 4) | (20, 3) | (52, 4) | (41, 4) | |
| - | p < 0.05 | p > 0.05 | p > 0.05 | p > 0.05 | |
| Bub1 Intensity (Bub1:ACA) | 100.00 ± 3.30 | 42.59 ± 0.85 | 24.24 ± 0.40 | 46.20 ± 0.98 | 68.50 ± 0.79 |
| (76, 4)‡ | (64, 4) | (116, 3) | (81, 4) | (124, 5) | |
| - | p < 0.05 | p < 0.001 | p < 0.001 | p > 0.05 | |
| BubR1 Intensity (BubR1:ACA) | 100.00 ± 0.40 | 63.67 ± 0.30 | 31.49 ± 1.24 | 89.27 ± 0.75 | 84.08 ± 0.49 |
| (121, 7)‡ | (115, 6) | (133, 4) | (176, 6) | (127, 5) | |
| - | p < 0.001 | p < 0.001 | p < 0.001 | p < 0.001 |
Quantitation of spindle length (pole to pole distance), inter-kinetochore distance and kinetochore localisation of Bub1 and BubR1 in DLD-1 cells treated with GSK-3 inhibitors.
* Values in parentheses (Number of cells)
‡ Values in parentheses (Number of kinetochores, Number of cells)
§ Kruskal-Wallis Test (Nonparametric ANOVA), Dunn's Multiple Comparisons Test.