Figure 3. Tethered HP1 Diminishes Modifications Characteristic of Active Chromatin.

(A) Representative fluorescent images of single DT40 PolyLacO-Vλ_R LacI-HP1 transfectants, stained with anti-LacI antibodies (left), DAPI (center), or merged image (right).

(B) Enrichment of AcH3 and AcH4 at ψVλ17_R in DT40 PolyLacO-Vλ_R GFP-LacI and DT40 PolyLacO-Vλ_R LacI-HP1 transfectants. Following ChIP, duplex PCR was carried out with Ova and ψVλ17_R primers. Enrichment is expressed relative to the total DNA input control ± standard deviation of four separate amplifications of increasing amounts of template DNA.

(C) Histogram showing enrichment of AcH3 and AcH4 at ψVλ17_R (from [B]) and the polɛ promoter in DT40 PolyLacO-Vλ_R GFP-LacI and DT40 PolyLacO-Vλ_R LacI-HP1 transfectants. Bars indicate standard deviation.