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. 2007 Sep 18;5(10):e246. doi: 10.1371/journal.pbio.0050246

Figure 4. Tethered HP1 Does Not Affect Igλ Expression.

Figure 4

(A) Relative enrichment of AcH3 and AcH4 at VλR in DT40 PolyLacO-VλR GFP-LacI and DT40 PolyLacO-VλR LacI-HP1 transfectants. Enrichment values were normalized to the DT40 PolyLacO-VλR GFP-LacI control. Bars indicate standard deviation of four separate amplifications of increasing amounts of template DNA.

(B) Relative intensity of sIgM expression in DT40 PolyLacO-λR GFP-LacI and DT40 PolyLacO-λR LacI-HP1 transfectants cultured in the presence or absence of 250 μM IPTG. sIgM levels were quantitated by measuring intensity of staining with mouse anti-chicken IgM antibody, and normalized to the level in DT40 PolyLacO-λR GFP-LacI transfectants. Details as in (A).

(C) Relative levels of VλR transcripts in DT40 PolyLacO-λR GFP-LacI and DT40 PolyLacO-λR LacI-HP1 transfectants. Transcript levels were quantitated by reverse transcriptase–PCR and normalized to the level in DT40 PolyLacO-λR GFP-LacI transfectants. Details as in (A).