Figure 5. Tethered HP1 Decreases Histone Acetylation throughout the ψVλ Array.

(A) Summary of a ChIP experiment, assaying N-terminal acetylation of histone H3 in chromatin from the DT40 PolyLacO-Vλ_R LacI-HP1 cell line cultured for 3 d in the presence or absence of 250 μM IPTG. ChIP enrichment values (see Material and Methods) were normalized to values obtained from a parallel analysis of chromatin from DT40 PolyLacO-Vλ_R GFP-LacI cells. Bars indicate standard deviation of four separate amplifications of increasing amounts of template DNA.

(B) Summary of a ChIP experiment, assaying N-terminal acetylation of histone H4 in chromatin from the DT40 PolyLacO-Vλ_R LacI-HP1 cell line cultured for 3 d in the presence or absence of 250 μM IPTG. Details as in (A).