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. 1984 Aug;50(2):179–183. doi: 10.1038/bjc.1984.160

Neurotensin in human small cell lung carcinoma.

M Goedert, J G Reeve, P C Emson, N M Bleehen
PMCID: PMC1976878  PMID: 6087865

Abstract

High levels of neurotensin-like immunoreactivity were found in human small cell lung carcinoma lines. No immunoreactivity was present in non-small cell carcinoma lines and only low amounts in postmortem human lung tissue. The immunoreactive material co-eluted with synthetic neurotensin on two different chromatographic systems. No evidence was obtained for the presence of specific neurotensin binding sites in any of the small cell carcinoma lines examined. The results suggest that small lung cell carcinoma lines may be useful for studying the biosynthesis of human neurotensin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araki K., Tachibana S., Uchiyama M., Nakajima T., Yasuhara T. Isolation and structure of a new active peptide "Xenopsin" on the smooth muscle, especially on a strip of fundus from a rat stomach, from the skin of Xenopus laevis. Chem Pharm Bull (Tokyo) 1973 Dec;21(12):2801–2804. doi: 10.1248/cpb.21.2801. [DOI] [PubMed] [Google Scholar]
  2. Blackburn A. M., Bryant M. G., Adrian T. E., Bloom S. R. Pancreatic tumours produce neurotensin. J Clin Endocrinol Metab. 1981 Apr;52(4):820–822. doi: 10.1210/jcem-52-4-820. [DOI] [PubMed] [Google Scholar]
  3. Carraway R., Leeman S. E. The isolation of a new hypotensive peptide, neurotensin, from bovine hypothalami. J Biol Chem. 1973 Oct 10;248(19):6854–6861. [PubMed] [Google Scholar]
  4. Emson P. C., Goedert M., Horsfield P., Rioux F., St Pierre S. The regional distribution and chromatographic characterisation of neurotensin-like immunoreactivity in the rat central nervous system. J Neurochem. 1982 Apr;38(4):992–999. doi: 10.1111/j.1471-4159.1982.tb05340.x. [DOI] [PubMed] [Google Scholar]
  5. Feurle G. E., Helmstaedter V., Tischbirek K., Carraway R., Forssmann W. G., Grube D., Röher H. D. A multihormonal tumor of the pancreas producing neurotensin. Dig Dis Sci. 1981 Dec;26(12):1125–1133. doi: 10.1007/BF01295980. [DOI] [PubMed] [Google Scholar]
  6. Gazdar A. F., Carney D. N., Russell E. K., Sims H. L., Baylin S. B., Bunn P. A., Jr, Guccion J. G., Minna J. D. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 1980 Oct;40(10):3502–3507. [PubMed] [Google Scholar]
  7. Goedert M., Mantyh P. W., Hunt S. P., Emson P. C. Mosaic distribution of neurotensin-like immunoreactivity in the cat striatum. Brain Res. 1983 Sep 5;274(1):176–179. doi: 10.1016/0006-8993(83)90536-x. [DOI] [PubMed] [Google Scholar]
  8. Greco F. A., Oldham R. K. Current concepts in cancer: small-cell lung cancer. N Engl J Med. 1979 Aug 16;301(7):355–358. doi: 10.1056/NEJM197908163010704. [DOI] [PubMed] [Google Scholar]
  9. Gu J., Islam K. N., Polak J. M. Repeated application of first-layer antiserum improves immunofluorescence staining: a modification of the indirect immunofluorescence staining procedure. Histochem J. 1983 May;15(5):475–482. doi: 10.1007/BF01002701. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Leeman S. E., Carraway R. E. Neurotensin: discovery, isolation, characterization, synthesis and possible physiological roles. Ann N Y Acad Sci. 1982;400:1–16. doi: 10.1111/j.1749-6632.1982.tb31557.x. [DOI] [PubMed] [Google Scholar]
  12. Moody T. W., Pert C. B., Gazdar A. F., Carney D. N., Minna J. D. High levels of intracellular bombesin characterize human small-cell lung carcinoma. Science. 1981 Dec 11;214(4526):1246–1248. doi: 10.1126/science.6272398. [DOI] [PubMed] [Google Scholar]
  13. Pearse A. G. The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J Histochem Cytochem. 1969 May;17(5):303–313. doi: 10.1177/17.5.303. [DOI] [PubMed] [Google Scholar]
  14. Richardson R. L., Greco F. A., Oldham R. K., Liddle G. W. Tumor products and potential markers in small cell lung cancer. Semin Oncol. 1978 Sep;5(3):253–262. [PubMed] [Google Scholar]
  15. Sporn M. B., Todaro G. J. Autocrine secretion and malignant transformation of cells. N Engl J Med. 1980 Oct 9;303(15):878–880. doi: 10.1056/NEJM198010093031511. [DOI] [PubMed] [Google Scholar]
  16. Theodorsson-Norheim E., Oberg K., Rosell S., Boström H. Neurotensinlike immunoreactivity in plasma and tumor tissue from patients with endocrine tumors of the pancreas and gut. Gastroenterology. 1983 Oct;85(4):881–889. [PubMed] [Google Scholar]
  17. Tischler A. S., Lee Y. C., Slayton V. W., Bloom S. R. Content and release of neurotensin in PC12 pheochromocytoma cell cultures: modulation by dexamethasone and nerve growth factor. Regul Pept. 1982 May;3(5-6):415–421. doi: 10.1016/0167-0115(82)90064-7. [DOI] [PubMed] [Google Scholar]
  18. Wood J. R., Wood S. M., Lee Y. C., Bloom S. R. Neurotensin-secreting carcinoma of the bronchus. Postgrad Med J. 1983 Jan;59(687):46–47. doi: 10.1136/pgmj.59.687.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wood S. M., Wood J. R., Ghatei M. A., Lee Y. C., O'Shaughnessy D., Bloom S. R. Bombesin, somatostatin and neurotensin-like immunoreactivity in bronchial carcinoma. J Clin Endocrinol Metab. 1981 Dec;53(6):1310–1312. doi: 10.1210/jcem-53-6-1310. [DOI] [PubMed] [Google Scholar]
  20. Yamaguchi K., Abe K., Kameya T., Adachi I., Taguchi S., Otsubo K., Yanaihara N. Production and molecular size heterogeneity of immunoreactive gastrin-releasing peptide in fetal and adult lungs and primary lung tumors. Cancer Res. 1983 Aug;43(8):3932–3939. [PubMed] [Google Scholar]
  21. Zeytinoğlu F. N., Gagel R. F., Tashjian A. H., Jr, Hammer R. A., Leeman S. E. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3741–3745. doi: 10.1073/pnas.77.6.3741. [DOI] [PMC free article] [PubMed] [Google Scholar]

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