Figure 3.

Transformation experiments. (A) E4orf6 cooperates with E1A to promote focus formation. Primary BRK cells were transfected with the indicated amounts of plasmids, and morphologically transformed colonies were scored 3 weeks after transfection. The mean number of dense foci from three independent experiments is presented. (B) E4orf6 cooperates with E1A and E1B proteins to enhance the number of cell transformants. Focus-forming activity is represented as a percentage of pAd5 XhoI-C activity. The average number of foci for pAd5 XhoI-C was 33 in four independent experiments.