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. 1997 Feb 18;94(4):1212–1217. doi: 10.1073/pnas.94.4.1212

Figure 4.

Figure 4

Monoclonal antibody C130 derived from a P2P-R cDNA fusion protein detects native P2P. Rapidly growing undifferentiated 3T3T total (T) cellular extracts (100 μg per lane) or nuclear (N) extracts (40 μg per lane) were separated on SDS/10% polyacrylamide gels and transferred to nitrocellulose membranes. Blots were probed with monoclonal antibody AC88 to detect native P2P proteins or with the P2P-R cDNA-derived monoclonal antibody C130. The locations of P2P proteins and hsp90 are indicated. Size standards are shown in kilodaltons.