Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1985 Jul;52(1):21–29. doi: 10.1038/bjc.1985.143

The effects of alpha and gamma interferons on human lung cancer cells grown in vitro or as xenografts in nude mice.

P R Twentyman, P Workman, K A Wright, N M Bleehen
PMCID: PMC1977166  PMID: 3925974

Abstract

We have compared the effects of alpha and recombinant gamma interferons (IFNs) on the growth of human lung cancer cell lines in vitro. There was a diversity of response amongst the lines studied, the most sensitive being COR-L23 (a large cell anaplastic carcinoma line) and POC (a small cell line). In these two lines, IFN-gamma was found to be more potent than IFN-alpha. During cell growth of line POC in the presence of IFN-gamma no significant shift in cell cycle distribution occurred. When lines COR-L23 and POC were grown as xenograft tumours in nude mice, daily injection of 4 X 10(5) units per mouse per day of IFN-gamma produced no discernible retardation of tumour growth.

Full text

PDF
21

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams A., Strander H., Cantell K. Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. J Gen Virol. 1975 Aug;28(2):207–217. doi: 10.1099/0022-1317-28-2-207. [DOI] [PubMed] [Google Scholar]
  2. Bakhanashvili M., Wreschner D. H., Salzberg S. Specific antigrowth effect of interferon on mouse cells transformed by murine sarcoma virus. Cancer Res. 1983 Mar;43(3):1289–1294. [PubMed] [Google Scholar]
  3. Balkwill F. R., Moodie E. M., Freedman V., Fantes K. H. Human interferon inhibits the growth of established human breast tumours in the nude mouse. Int J Cancer. 1982 Aug 15;30(2):231–235. doi: 10.1002/ijc.2910300217. [DOI] [PubMed] [Google Scholar]
  4. Balkwill F., Watling D., Taylor-Papadimitriou J. Inhibition by lymphoblastoid interferon of growth of cells derived from the human breast. Int J Cancer. 1978 Sep 15;22(3):258–265. doi: 10.1002/ijc.2910220307. [DOI] [PubMed] [Google Scholar]
  5. Cantell K., Hirvonen S. Preparation of human leukocyte interferon for clinical use. Tex Rep Biol Med. 1977;35:138–144. [PubMed] [Google Scholar]
  6. Carney D. N., Bunn P. A., Jr, Gazdar A. F., Pagan J. A., Minna J. D. Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung. Proc Natl Acad Sci U S A. 1981 May;78(5):3185–3189. doi: 10.1073/pnas.78.5.3185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clutterbuck R. D., Millar J. L., Alexander P. Failure of high doses of alpha interferon to affect the growth of human carcinoma, melanoma, and myeloid leukaemia xenografts. Br J Cancer. 1983 Sep;48(3):445–447. doi: 10.1038/bjc.1983.212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Creasey A. A., Bartholomew J. C., Merigan T. C. The importance of G0 in the site of action of interferon in the cell cycle. Exp Cell Res. 1981 Jul;134(1):155–160. doi: 10.1016/0014-4827(81)90472-9. [DOI] [PubMed] [Google Scholar]
  9. Gewert D. R., Shah S., Clemens M. J. Inhibition of cell division by interferons: Changes in the transport and intracellular metabolism of thymidine in human lymphoblastoid (Daudi) cells. Eur J Biochem. 1981 Jun 1;116(3):487–492. doi: 10.1111/j.1432-1033.1981.tb05362.x. [DOI] [PubMed] [Google Scholar]
  10. Gutterman J. U., Rosenblum M. G., Rios A., Fritsche H. A., Quesada J. R. Pharmacokinetic study of partially pure gamma-interferon in cancer patients. Cancer Res. 1984 Sep;44(9):4164–4171. [PubMed] [Google Scholar]
  11. Jones D. H., Bleehen N. M., Slater A. J., George P. J., Walker J. R., Dixon A. K. Human lymphoblastoid interferon in the treatment of small cell lung cancer. Br J Cancer. 1983 Mar;47(3):361–366. doi: 10.1038/bjc.1983.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Killander D., Lindahl P., Lundin L., Leary P., Gresser I. Relationship between the enhanced expression of histocompatibility antigens on interferon-treated L 1210 cells and their position in the cell cycle. Eur J Immunol. 1976 Jan;6(1):56–59. doi: 10.1002/eji.1830060112. [DOI] [PubMed] [Google Scholar]
  13. Lundblad D., Lundgren E. Block of glioma cell line in S by interferon. Int J Cancer. 1981 Jun 15;27(6):749–754. doi: 10.1002/ijc.2910270604. [DOI] [PubMed] [Google Scholar]
  14. Lundgren E., Larsson I., Miörner H., Strannegård O. Effects of leukocyte and fibroblast interferon on events in the fibroblast cell cycle. J Gen Virol. 1979 Mar;42(3):589–595. doi: 10.1099/0022-1317-42-3-589. [DOI] [PubMed] [Google Scholar]
  15. PAUCKER K., CANTELL K., HENLE W. Quantitative studies on viral interference in suspended L cells. III. Effect of interfering viruses and interferon on the growth rate of cells. Virology. 1962 Jun;17:324–334. doi: 10.1016/0042-6822(62)90123-x. [DOI] [PubMed] [Google Scholar]
  16. Roos G., Leanderson T., Lundgren E. Interferon-induced cell cycle changes in human hematopoietic cell lines and fresh leukemic cells. Cancer Res. 1984 Jun;44(6):2358–2362. [PubMed] [Google Scholar]
  17. Stewart W. E., 2nd Distinct molecular species of interferons. Virology. 1974 Sep;61(1):80–86. doi: 10.1016/0042-6822(74)90243-8. [DOI] [PubMed] [Google Scholar]
  18. Taylor I. W. A rapid single step staining technique for DNA analysis by flow microfluorimetry. J Histochem Cytochem. 1980 Sep;28(9):1021–1024. doi: 10.1177/28.9.6157714. [DOI] [PubMed] [Google Scholar]
  19. Twentyman P. R. Growth delay in small EMT6 spheroids induced by cytotoxic drugs and its modification by misonidazole pretreatment under hypoxic conditions. Br J Cancer. 1982 Apr;45(4):565–570. doi: 10.1038/bjc.1982.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Twentyman P. R., Kallman R. F., Brown J. M. The effect of time between X-irradiation and chemotherapy on the growth of three solid mouse tumors--I. Adriamycin. Int J Radiat Oncol Biol Phys. 1979 Aug;5(8):1255–1260. doi: 10.1016/0360-3016(79)90649-7. [DOI] [PubMed] [Google Scholar]
  21. Twentyman P. R. Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival. Br J Cancer. 1980 Aug;42(2):297–304. doi: 10.1038/bjc.1980.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Watanabe Y., Sokawa Y. Effect of interferon on the cell cycle of BALB/c 3T3 cells. J Gen Virol. 1978 Nov;41(2):411–415. doi: 10.1099/0022-1317-41-2-411. [DOI] [PubMed] [Google Scholar]
  23. Watson J. V. Enzyme kinetic studies in cell populations using fluorogenic substrates and flow cytometric techniques. Cytometry. 1980 Sep;1(2):143–151. doi: 10.1002/cyto.990010209. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES