Table 1.
Plasmid | Independent transformants in U. maydis (a2Δb) | Pathogenicity symptoms*
|
Filamentous growth† | |
---|---|---|---|---|
Strong | Weak | |||
pbE1ΔHD-k-W2 | 20 | 0 | 0 | 3 |
pbE1-k-W2ΔHD | 23 | 0 | 0 | 3 |
pE1ΔHD-k-W2ΔHD | 15 | 0 | 0 | 2 |
Strong and weak pathogenicity symptoms scored as tumor formation and anthocyanin synthesis, respectively, were assayed as described.
Filamentous growth was assayed by spotting cultures of the respective transformant on charcoal plates (see Material and Methods).