Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1992 May;65(5):655–660. doi: 10.1038/bjc.1992.140

Transforming growth factor-beta mRNA expression and growth control of human ovarian carcinoma cells.

J M Bartlett 1, G J Rabiasz 1, W N Scott 1, S P Langdon 1, J F Smyth 1, W R Miller 1
PMCID: PMC1977402  PMID: 1586592

Abstract

The pattern of TGF beta expression and in vitro response to TGF beta has been defined in three ovarian carcinoma cell lines (PEO1, PEO4 and PEO14). Marked differences in both mRNA expression and growth responses were detected between the cell lines. All expressed mRNA for TGF beta 3, PEO1 and PEO4 but not PEO14 expressed mRNA for TGF beta 1, whereas PEO14 but not PEO1 and PEO4 expressed TGF beta 2. Growth of PEO14 cells in culture was markedly inhibited by both TGF beta 1 and beta 2. PEO1 cells were inhibited by TGF beta 1, but not TGF beta 2 whilst growth of PEO4 cells were not affected by exposure to either of these peptides. These data indicate that several elements of potential autocrine loops involving TGF beta's are present within ovarian cancer cells.

Full text

PDF
658

Images in this article

656Figure 1
on p.656
657Figure 2
on p.657
657Figure 3
on p.657

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adashi E. Y., Resnick C. E., Hernandez E. R., May J. V., Purchio A. F., Twardzik D. R. Ovarian transforming growth factor-beta (TGF beta): cellular site(s), and mechanism(s) of action. Mol Cell Endocrinol. 1989 Feb;61(2):247–256. doi: 10.1016/0303-7207(89)90136-6. [DOI] [PubMed] [Google Scholar]
  2. Akhurst R. J., Lehnert S. A., Gatherer D., Duffie E. The role of TGF beta in mouse development. Ann N Y Acad Sci. 1990;593:259–271. doi: 10.1111/j.1749-6632.1990.tb16117.x. [DOI] [PubMed] [Google Scholar]
  3. Arrick B. A., Korc M., Derynck R. Differential regulation of expression of three transforming growth factor beta species in human breast cancer cell lines by estradiol. Cancer Res. 1990 Jan 15;50(2):299–303. [PubMed] [Google Scholar]
  4. Arteaga C. L., Tandon A. K., Von Hoff D. D., Osborne C. K. Transforming growth factor beta: potential autocrine growth inhibitor of estrogen receptor-negative human breast cancer cells. Cancer Res. 1988 Jul 15;48(14):3898–3904. [PubMed] [Google Scholar]
  5. Barnard J. A., Lyons R. M., Moses H. L. The cell biology of transforming growth factor beta. Biochim Biophys Acta. 1990 Jun 1;1032(1):79–87. doi: 10.1016/0304-419x(90)90013-q. [DOI] [PubMed] [Google Scholar]
  6. Bonewald L. F., Mundy G. R. Role of transforming growth factor-beta in bone remodeling. Clin Orthop Relat Res. 1990 Jan;(250):261–276. [PubMed] [Google Scholar]
  7. Colletta A. A., Wakefield L. M., Howell F. V., Danielpour D., Baum M., Sporn M. B. The growth inhibition of human breast cancer cells by a novel synthetic progestin involves the induction of transforming growth factor beta. J Clin Invest. 1991 Jan;87(1):277–283. doi: 10.1172/JCI114983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Daly R. J., Darbre P. D. Cellular and molecular events in loss of estrogen sensitivity in ZR-75-1 and T-47-D human breast cancer cells. Cancer Res. 1990 Sep 15;50(18):5868–5875. [PubMed] [Google Scholar]
  9. Derynck R., Jarrett J. A., Chen E. Y., Eaton D. H., Bell J. R., Assoian R. K., Roberts A. B., Sporn M. B., Goeddel D. V. Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells. Nature. 1985 Aug 22;316(6030):701–705. doi: 10.1038/316701a0. [DOI] [PubMed] [Google Scholar]
  10. Frolik C. A., Wakefield L. M., Smith D. M., Sporn M. B. Characterization of a membrane receptor for transforming growth factor-beta in normal rat kidney fibroblasts. J Biol Chem. 1984 Sep 10;259(17):10995–11000. [PubMed] [Google Scholar]
  11. Kim I. C., Schomberg D. W. The production of transforming growth factor-beta activity by rat granulosa cell cultures. Endocrinology. 1989 Mar;124(3):1345–1351. doi: 10.1210/endo-124-3-1345. [DOI] [PubMed] [Google Scholar]
  12. Knabbe C., Lippman M. E., Wakefield L. M., Flanders K. C., Kasid A., Derynck R., Dickson R. B. Evidence that transforming growth factor-beta is a hormonally regulated negative growth factor in human breast cancer cells. Cell. 1987 Feb 13;48(3):417–428. doi: 10.1016/0092-8674(87)90193-0. [DOI] [PubMed] [Google Scholar]
  13. Knecht M., Feng P., Catt K. Bifunctional role of transforming growth factor-beta during granulosa cell development. Endocrinology. 1987 Apr;120(4):1243–1249. doi: 10.1210/endo-120-4-1243. [DOI] [PubMed] [Google Scholar]
  14. Langdon S. P., Lawrie S. S., Hay F. G., Hawkes M. M., McDonald A., Hayward I. P., Schol D. J., Hilgers J., Leonard R. C., Smyth J. F. Characterization and properties of nine human ovarian adenocarcinoma cell lines. Cancer Res. 1988 Nov 1;48(21):6166–6172. [PubMed] [Google Scholar]
  15. Lucas C., Bald L. N., Fendly B. M., Mora-Worms M., Figari I. S., Patzer E. J., Palladino M. A. The autocrine production of transforming growth factor-beta 1 during lymphocyte activation. A study with a monoclonal antibody-based ELISA. J Immunol. 1990 Sep 1;145(5):1415–1422. [PubMed] [Google Scholar]
  16. Magoffin D. A., Gancedo B., Erickson G. F. Transforming growth factor-beta promotes differentiation of ovarian thecal-interstitial cells but inhibits androgen production. Endocrinology. 1989 Oct;125(4):1951–1958. doi: 10.1210/endo-125-4-1951. [DOI] [PubMed] [Google Scholar]
  17. Marth C., Lang T., Koza A., Mayer I., Daxenbichler G. Transforming growth factor-beta and ovarian carcinoma cells: regulation of proliferation and surface antigen expression. Cancer Lett. 1990 Jun 15;51(3):221–225. doi: 10.1016/0304-3835(90)90106-8. [DOI] [PubMed] [Google Scholar]
  18. Massagué J., Cheifetz S., Boyd F. T., Andres J. L. TGF-beta receptors and TGF-beta binding proteoglycans: recent progress in identifying their functional properties. Ann N Y Acad Sci. 1990;593:59–72. doi: 10.1111/j.1749-6632.1990.tb16100.x. [DOI] [PubMed] [Google Scholar]
  19. Massagué J., Like B. Cellular receptors for type beta transforming growth factor. Ligand binding and affinity labeling in human and rodent cell lines. J Biol Chem. 1985 Mar 10;260(5):2636–2645. [PubMed] [Google Scholar]
  20. Nilsen-Hamilton M. Transforming growth factor-beta and its actions on cellular growth and differentiation. Curr Top Dev Biol. 1990;24:95–136. [PubMed] [Google Scholar]
  21. Noda M., Rodan G. A. Type beta transforming growth factor regulates expression of genes encoding bone matrix proteins. Connect Tissue Res. 1989;21(1-4):71–75. doi: 10.3109/03008208909049997. [DOI] [PubMed] [Google Scholar]
  22. Roberts A. B., Anzano M. A., Lamb L. C., Smith J. M., Sporn M. B. New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5339–5343. doi: 10.1073/pnas.78.9.5339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Roberts A. B., Anzano M. A., Wakefield L. M., Roche N. S., Stern D. F., Sporn M. B. Type beta transforming growth factor: a bifunctional regulator of cellular growth. Proc Natl Acad Sci U S A. 1985 Jan;82(1):119–123. doi: 10.1073/pnas.82.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Roberts A. B., Flanders K. C., Heine U. I., Jakowlew S., Kondaiah P., Kim S. J., Sporn M. B. Transforming growth factor-beta: multifunctional regulator of differentiation and development. Philos Trans R Soc Lond B Biol Sci. 1990 Mar 12;327(1239):145–154. doi: 10.1098/rstb.1990.0050. [DOI] [PubMed] [Google Scholar]
  25. Roberts A. B., Rosa F., Roche N. S., Coligan J. E., Garfield M., Rebbert M. L., Kondaiah P., Danielpour D., Kehrl J. H., Wahl S. M. Isolation and characterization of TGF-beta 2 and TGF-beta 5 from medium conditioned by Xenopus XTC cells. Growth Factors. 1990;2(2-3):135–147. doi: 10.3109/08977199009071500. [DOI] [PubMed] [Google Scholar]
  26. Roberts A. B., Sporn M. B. Regulation of endothelial cell growth, architecture, and matrix synthesis by TGF-beta. Am Rev Respir Dis. 1989 Oct;140(4):1126–1128. doi: 10.1164/ajrccm/140.4.1126. [DOI] [PubMed] [Google Scholar]
  27. Schwarz L. C., Wright J. A., Gingras M. C., Kondaiah P., Danielpour D., Pimentel M., Sporn M. B., Greenberg A. H. Aberrant TGF-beta production and regulation in metastatic malignancy. Growth Factors. 1990;3(2):115–127. doi: 10.3109/08977199009108274. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES