Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1992 Dec;66(6):1150–1154. doi: 10.1038/bjc.1992.425

Oestrogen receptor protein and mRNA in adenocarcinoma of the uterine cervix.

S M Ismail 1, G A Thomas 1, F A Ghandour 1, H G Davies 1, R Attanoos 1, E D Williams 1
PMCID: PMC1978005  PMID: 1457356

Abstract

We have investigated the oestrogen receptor (ER) status of 20 cervical adenocarcinomas by immunocytochemistry for ER protein and non-isotopic in situ hybridisation for ER mRNA. Both methods, which are applicable to paraffin sections, were developed and validated in breast carcinomas with known ER content. Six cervical adenocarcinomas contained immunocytochemically demonstrable ER protein; all contained ER mRNA, but staining was less intense in poorly differentiated areas of four tumours. This disparity between protein and mRNA detection needs further investigation as does the possibility that oestrogens may play a role in the pathogenesis of cervical adenocarcinoma.

Full text

PDF
1150

Images in this article

1151Figure 1
on p.1151
1152Figure 2
on p.1152
1152Figure 3
on p.1152
1153Figure 4
on p.1153

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen J., Orntoft T., Poulsen H. S. Semiquantitative oestrogen receptor assay in formalin-fixed paraffin sections of human breast cancer tissue using monoclonal antibodies. Br J Cancer. 1986 May;53(5):691–694. doi: 10.1038/bjc.1986.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boultwood J., Wynford-Thomas D., Richards G. P., Craig R. K., Williams E. D. In-situ analysis of calcitonin and CGRP expression in medullary thyroid carcinoma. Clin Endocrinol (Oxf) 1990 Sep;33(3):381–390. doi: 10.1111/j.1365-2265.1990.tb00503.x. [DOI] [PubMed] [Google Scholar]
  3. Cano A., Serra V., Rivera J., Monmeneu R., Marzo C. Expression of estrogen receptors, progesterone receptors, and an estrogen receptor-associated protein in the human cervix during the menstrual cycle and menopause. Fertil Steril. 1990 Dec;54(6):1058–1064. doi: 10.1016/s0015-0282(16)54005-9. [DOI] [PubMed] [Google Scholar]
  4. Cheng L., Binder S. W., Fu Y. S., Lewin K. J. Demonstration of estrogen receptors by monoclonal antibody in formalin-fixed breast tumors. Lab Invest. 1988 Mar;58(3):346–353. [PubMed] [Google Scholar]
  5. Chilvers C., Mant D., Pike M. C. Cervical adenocarcinoma and oral contraceptives. Br Med J (Clin Res Ed) 1987 Dec 5;295(6611):1446–1447. doi: 10.1136/bmj.295.6611.1446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eick D., Piechaczyk M., Henglein B., Blanchard J. M., Traub B., Kofler E., Wiest S., Lenoir G. M., Bornkamm G. W. Aberrant c-myc RNAs of Burkitt's lymphoma cells have longer half-lives. EMBO J. 1985 Dec 30;4(13B):3717–3725. doi: 10.1002/j.1460-2075.1985.tb04140.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farquharson M., Harvie R., McNicol A. M. Detection of messenger RNA using a digoxigenin end labelled oligodeoxynucleotide probe. J Clin Pathol. 1990 May;43(5):424–428. doi: 10.1136/jcp.43.5.424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ford L. C., Berek J. S., Lagasse L. D., Hacker N. F., Heins Y. L., DeLange R. J. Estrogen and progesterone receptor sites in malignancies of the uterine cervix, vagina, and vulva. Gynecol Oncol. 1983 Feb;15(1):27–31. doi: 10.1016/0090-8258(83)90113-0. [DOI] [PubMed] [Google Scholar]
  9. Fuqua S. A., Fitzgerald S. D., Chamness G. C., Tandon A. K., McDonnell D. P., Nawaz Z., O'Malley B. W., McGuire W. L. Variant human breast tumor estrogen receptor with constitutive transcriptional activity. Cancer Res. 1991 Jan 1;51(1):105–109. [PubMed] [Google Scholar]
  10. Gao Y. L., Twiggs L. B., Leung B. S., Yu W. C., Potish R. A., Okagaki T., Adcock L. L., Prem K. A. Cytoplasmic estrogen and progesterone receptors in primary cervical carcinoma: clinical and histopathologic correlates. Am J Obstet Gynecol. 1983 Jun 1;146(3):299–306. doi: 10.1016/0002-9378(83)90752-4. [DOI] [PubMed] [Google Scholar]
  11. Graham D. M., Jin L., Lloyd R. V. Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization. Am J Surg Pathol. 1991 May;15(5):475–485. doi: 10.1097/00000478-199105000-00008. [DOI] [PubMed] [Google Scholar]
  12. Green S., Walter P., Kumar V., Krust A., Bornert J. M., Argos P., Chambon P. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature. 1986 Mar 13;320(6058):134–139. doi: 10.1038/320134a0. [DOI] [PubMed] [Google Scholar]
  13. Harding M., McIntosh J., Paul J., Symonds R. P., Reed N., Habeshaw T., Stewart M., Leake R. E. Oestrogen and progesterone receptors in carcinoma of the cervix. Clin Oncol (R Coll Radiol) 1990 Nov;2(6):313–317. doi: 10.1016/s0936-6555(05)80992-6. [DOI] [PubMed] [Google Scholar]
  14. Hunter R. E., Longcope C., Keough P. Steroid hormone receptors in carcinoma of the cervix. Cancer. 1987 Aug 1;60(3):392–396. doi: 10.1002/1097-0142(19870801)60:3<392::aid-cncr2820600318>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  15. Kupryjańczyk J., Möller P. Estrogen receptor distribution in the normal and pathologically changed human cervix uteri: an immunohistochemical study with use of monoclonal anti-ER antibody. Int J Gynecol Pathol. 1988;7(1):75–85. doi: 10.1097/00004347-198803000-00008. [DOI] [PubMed] [Google Scholar]
  16. Martin J. D., Hähnel R., McCartney A. J., De Klerk N. The influence of estrogen and progesterone receptors on survival in patients with carcinoma of the uterine cervix. Gynecol Oncol. 1986 Mar;23(3):329–335. doi: 10.1016/0090-8258(86)90134-4. [DOI] [PubMed] [Google Scholar]
  17. Martin J. D., Hähnel R. Oestrogen receptor studies in carcinoma of the endometrium, carcinoma of the uterine cervix and other gynaecological malignancies. Aust N Z J Obstet Gynaecol. 1978 Feb;18(1):55–59. doi: 10.1111/j.1479-828x.1978.tb00012.x. [DOI] [PubMed] [Google Scholar]
  18. Matlashewski G., Banks L., Pim D., Crawford L. Analysis of human p53 proteins and mRNA levels in normal and transformed cells. Eur J Biochem. 1986 Feb 3;154(3):665–672. doi: 10.1111/j.1432-1033.1986.tb09449.x. [DOI] [PubMed] [Google Scholar]
  19. Murphy L. C. Estrogen receptor variants in human breast cancer. Mol Cell Endocrinol. 1990 Dec 3;74(2):C83–C86. doi: 10.1016/0303-7207(90)90109-l. [DOI] [PubMed] [Google Scholar]
  20. Nonogaki H., Fujii S., Konishi I., Nanbu Y., Ozaki S., Ishikawa Y., Mori T. Estrogen receptor localization in normal and neoplastic epithelium of the uterine cervix. Cancer. 1990 Dec 15;66(12):2620–2627. doi: 10.1002/1097-0142(19901215)66:12<2620::aid-cncr2820661226>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  21. Pelletier G., Liao N., Follea N., Govindan M. V. Distribution of estrogen receptors in the rat pituitary as studied by in situ hybridization. Mol Cell Endocrinol. 1988 Mar;56(1-2):29–33. doi: 10.1016/0303-7207(88)90005-6. [DOI] [PubMed] [Google Scholar]
  22. Peters R. K., Chao A., Mack T. M., Thomas D., Bernstein L., Henderson B. E. Increased frequency of adenocarcinoma of the uterine cervix in young women in Los Angeles County. J Natl Cancer Inst. 1986 Mar;76(3):423–428. [PubMed] [Google Scholar]
  23. Ponglikitmongkol M., Green S., Chambon P. Genomic organization of the human oestrogen receptor gene. EMBO J. 1988 Nov;7(11):3385–3388. doi: 10.1002/j.1460-2075.1988.tb03211.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Potish R. A., Twiggs L. B., Adcock L. L., Prem K. A., Savage J. E., Leung B. S. Prognostic importance of progesterone and estrogen receptors in cancer of the uterine cervix. Cancer. 1986 Oct 15;58(8):1709–1713. doi: 10.1002/1097-0142(19861015)58:8<1709::aid-cncr2820580822>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  25. Scheffner M., Werness B. A., Huibregtse J. M., Levine A. J., Howley P. M. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell. 1990 Dec 21;63(6):1129–1136. doi: 10.1016/0092-8674(90)90409-8. [DOI] [PubMed] [Google Scholar]
  26. Schwartz S. M., Weiss N. S. Increased incidence of adenocarcinoma of the cervix in young women in the United States. Am J Epidemiol. 1986 Dec;124(6):1045–1047. doi: 10.1093/oxfordjournals.aje.a114474. [DOI] [PubMed] [Google Scholar]
  27. Wakefield E. A., Wells M. Histochemical study of endocervical glycoproteins throughout the normal menstrual cycle and adjacent to cervical intraepithelial neoplasia. Int J Gynecol Pathol. 1985;4(3):230–239. doi: 10.1097/00004347-198509000-00007. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES