. Author manuscript; available in PMC: 2007 Sep 17.

Published in final edited form as: Structure. 2007 Mar;15(3):363–376. doi: 10.1016/j.str.2007.01.018

Table 2.

Amino acid substitutions in P. aeruginosa PilT.

Residue changed	A.a. #	Morph	Twitching	Location
WT	n/a	F	yes	n/a
D17G	29	S	no	NTD_n:CTD_n+1
H222R	235	S	no	His Box
K297E	310	S	no	αJ-αK loop
R82Q	95	S	no	β5 Arg
R82S	95	S	no	β5 Arg
R97Q	110	S	no	β5 Arg
R97S	110	S	no	β5 Arg
R194Q	207	S	no	Arg Finger
R194S	207	S	no	Arg Finger

Each of the P. aeruginosa PilT variants listed (with corresponding A. aeolicus amino acid numbers) was isolated in a random screen or created by site-directed mutagenesis (R82/R97/R194 variants). Colony morphology of P. aeruginosa expressing PilT variants is tabulated as F (fingered, like wild-type strain PAK), or S (smooth like pilT). Twitching motility at an agar-plastic surface was not observed for any mutant.